There’s a trans character in the 1994 American comedy Naked Gun 33 1/3: The Final Insult, and the film reveals her trans status in a scene clearly meant as a parody of 1992′s The Crying Game, down to the set dressing. As trans woman Tanya (Anna Nicole Smith) undresses in front of Frank (Leslie Nielsen), we switch from Frank’s eyeline to one that shows him in profile and casts her out of frame. Only her shadow remains.
It is, effectively, her shadow with which he interacts next.
Her penis outs her to Frank and to the audience. Frank bolts from the scene, and Tanya, who did at least have some appearances and lines in the film up to this point, is now gone. She will not appear in the film again. Unlike the other two villains, who are killed, she is defeated merely by the existence of her genitalia.